Tag

Part:BBa_K2323019:Design

Designed by: Aurore Dupin   Group: iGEM17_Munich   (2017-10-30)


pdt2B degradation tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

This degradation tag is taken from Cameron & Collins, Tunable protein degradation in bacteria, Nature biotechnology, 32(12), December 2014. It can be degraded at a slow rate (0.0069/min) by the ClpP machinery of E.coli, but also at a faster rate by an exogenous protease (mf-lon).

References

Cameron & Collins, Tunable protein degradation in bacteria, Nature biotechnology, 32(12), December 2014